| 31 | | Pitlick, Tyler | | R | 6'1 | 190 | R | 11/01/91 | WCHA | 99 | 99 | 62 | 99 | 99 | 99 |
| 32 | The pick re-acquired by Toronto was traded to the Boston Bruins as the result of a trade on September 18, 2009 that sent this pick along with first-round picks in 2010 & 2011 to Boston in exchange for Phil Kessel. | Knight, Jared | | C | 5'11 | 190 | R | 01/15/92 | OHL | 99 | 99 | 101 | 99 | 99 | 99 |
| 33 | | McFarland, John | | C | 6'0 | 192 | R | 04/01/92 | OHL | 6 | 20 | 21 | 6 | 99 | 6 |
| 34 | | Smith, Dalton | | L | 6'1 | 195 | L | 06/30/92 | OHL | 99 | 99 | 72 | 99 | 99 | 99 |
| 35 | | Rensfeldt, Ludvig | | C | 6'3 | 194 | L | 01/29/92 | SWE | 99 | 99 | 101 | 99 | 99 | 99 |
| 36 | The Tampa Bay Lightning's second-round pick will go to the Florida Panthers as the result of a trade on March 3, 2010 that sent Dennis Seidenberg and Matthew Bartkowski to the Boston Bruins in exchange for Craig Weller, Byron Bitz and this pick. | Petrovic, Alex | | D | 6'4 | 193 | R | 03/03/92 | WHL | 99 | 99 | 34 | 3 | 99 | 99 |
| 37 | | Faulk, Justin | | D | 5'11 | 185 | R | 03/20/92 | USHL | 99 | 99 | 101 | 4 | 99 | 99 |
| 38 | The New Jersey Devils' first-round pick will go to the Atlanta Thrashers as the result of a trade on February 4, 2010 that sent Ilya Kovalchuk, Anssi Salmela and a second round pick in 2010 to New Jersey in exchange for Niclas Bergfors, Johnny Oduya, Patrice Cormier, a second round pick in 2010 and this pick. | Merrill, Jon | | D | 6'4 | 185 | L | 02/03/92 | USHL | 11 | 99 | 11 | 7 | 99 | 99 |
| 39 | | Kabanov, Kirill | | L | 6'2 | 177 | L | 07/16/92 | QMJHL | 4 | 5 | 10 | 2 | 99 | 4 |
| 40 | | Thomas, Christian | | R | 5'09 | 171 | R | 05/26/92 | OHL | 99 | 99 | 101 | 25 | 99 | 99 |
| 41 | | Nemeth, Patrik | | D | 6'3 | 208 | L | 02/08/92 | SWE | 99 | 99 | 91 | 99 | 99 | 99 |
| 42 | | Smith-Pelly, Devante | | L | 5'11 | 180 | L | 06/14/92 | OHL | 99 | 99 | 51 | 99 | 99 | 99 |
| 43 | The Philadelphia Flyers' second round pick will go to the Los Angeles Kings due to a trade on July 1, 2008 that sent Patrik Hersley and Ned Lukacevic to Philadelphia in exchange for Denis Gauthier and this pick. | Ross, Brad | | L | 6'0 | 173 | L | 05/28/92 | WHL | 99 | 99 | 101 | 13 | 99 | 99 |
| 44 | | Kitsyn, Maxim | | L | 6'2 | 179 | R | 12/24/91 | KHL | 27 | 99 | 16 | 99 | 99 | 99 |
| 45 | The Calgary Flames' second round pick will go to the Chicago Blackhawks as a result of a trade on July 1, 2008 that sent Rene Bourque to Calgary in exchange for this pick (being conditional at the time of the trade). The condition : Calgary chooses to trade a pick in either 2009 or 2010 : has been converted on March 4, 2009 when Calgary traded the 2009 pick in question to the Colorado Avalanche. | Galiev, Stanislav | | L | 6'1 | 177 | L | 01/17/92 | QMJHL | 19 | 13 | 20 | 7 | 99 | 99 |
| 46 | | Pulkkinen, Teemu | | R | 5'10 | 178 | R | 01/02/92 | FIN | 22 | 99 | 19 | 99 | 99 | 99 |
| 47 | | Johns, Stephen | | D | 6'4 | 205 | R | 04/18/92 | NTDP | 99 | 19 | 13 | 3 | 99 | 99 |
| 48 | The Ottawa Senators' second-round pick will go to the Carolina Hurricanes as the result of a trade on February 12, 2010 that sent Matt Cullen to Ottawa in exchange for Alexandre R. Picard and this pick. | Berglund, Jacob | | L | 6'1 | 200 | L | 11/17/91 | SWE | 99 | 99 | 68 | 99 | 99 | 99 |
| 49 | | Beukeboom, Brock | | D | 6'1 | 202 | R | 04/01/92 | OHL | 99 | 99 | 27 | 12 | 99 | 99 |
| 50 | The Buffalo Sabres' second round pick will go to the San Jose Sharks due to a trade on July 4, 2008 that sent Craig Rivet and a 7th round pick in 2010 to Buffalo in exchange for a 2nd round pick in 2009 and this pick. | Moffatt, Luke | | R | 5'11 | 185 | R | 06/11/92 | USHL | 99 | 99 | 40 | 13 | 99 | 99 |
| 51 | The Nashville Predators' second-round pick will go to the Edmonton Oilers as the result of a trade on March 1, 2010 that sent Denis Grebeshkov to Nashville in exchange for this pick. | Ohman, Victor | | R | 6'1 | 190 | L | 04/07/92 | SWE | 99 | 99 | 31 | 99 | 99 | 99 |
| 52 | | Martindale, Ryan | | C | 6'3 | 190 | L | 10/27/91 | OHL | 99 | 99 | 48 | 7 | 99 | 99 |
| 53 | The Pittsburgh Penguins' second-round pick will go to the Florida Panthers as the result of a trade on March 1, 2010 that sent Jordan Leopold to Pittsburgh in exchange for this pick. | Pettersson, Adam | | C | 6'0 | 185 | L | 01/13/92 | SWE | 99 | 99 | 35 | 99 | 99 | 99 |
| 54 | | Sundher, Kevin | | C | 5'11 | 186 | L | 01/18/92 | WHL | 99 | 99 | 44 | 18 | 99 | 99 |
| 55 | The New Jersey Devils' first-round pick will go to the Atlanta Thrashers as the result of a trade on February 4, 2010 that sent Ilya Kovalchuk, Anssi Salmela and a second round pick in 2010 to New Jersey in exchange for Niclas Bergfors, Johnny Oduya, Patrice Cormier, a second round pick in 2010 and this pick. | Toffoli, Tyler | | R | 6'0 | 185 | R | 04/24/92 | OHL | 99 | 99 | 37 | 14 | 99 | 99 |
| 56 | The Washington Capitals' second-round pick will go to the Minnesota Wild as the result of a trade on March 3, 2010 that sent Eric Belanger to Washington in exchange for this pick. | Mattson, Nick | | D | 6'0 | 176 | L | 10/25/91 | USHL | 99 | 99 | 45 | 11 | 99 | 99 |
| 57 | The Vancouver Canucks' second-round pick will go to the Columbus Blue Jackets as the result of a trade on March 3, 2010 that sent Raffi Torres to the Buffalo Sabres in exchange for Nathan Paetsch and this pick. | Lindberg, Oscar | | L | 6'0 | 187 | L | 10/29/91 | SWE | 99 | 99 | 39 | 99 | 99 | 99 |
| 58 | | Trotman, Zach | | D | 6'4 | 202 | R | 08/26/90 | NCAA | 99 | 99 | 33 | 99 | 99 | 99 |
| 59 | | Liljegren, Felix | | R | 6'4 | 225 | L | 07/27/92 | SWE | 99 | 99 | 55 | 99 | 99 | 99 |
| 60 | The San Jose Sharks' second-round pick will go to the Ottawa Senators as the result of a trade on September 12, 2009 that sent Dany Heatley and a fifth-round pick in 2010 to San Jose in exchange for Milan Michalek, Jonathan Cheechoo and this pick. | Duffy, Ben | | C | 5'10 | 178 | R | 03/03/92 | QMJHL | 99 | 99 | 46 | 99 | 99 | 99 |